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| 品牌 | StemRD/美國 |
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產品英文名稱:WNT-5a,W5A-M-025，Mouse，25ug
產品中文名稱:鼠重組WNT-5a蛋白
產品信息
Wnt糖蛋白是存在于多種生物體內的一種細胞外配體，Wnt作為形態發生素通過激發細胞內遠離信號發送區域的濃度依賴反應控制胚胎形態發育。Wnt通路調節動物發育過程中的多個重要環節，例如細胞增殖、細胞遷徙及細胞分化。Wnt蛋白通過自分泌或旁分泌作用與位于細胞膜上的受體相結合，激活細胞內信號通路調節靶基因的表達，對細胞的增殖、分化、遷移、極性化和凋亡起到重要作用。
WNT-5a,Mouse產品介紹:
wnt信號轉導通路因其啟動蛋白為Wnt蛋白而得名，在胚胎發育、細胞增殖及分化等方面發揮重要作用，其異常活化與人類多種腫瘤的發生密切相關。研究顯示，Wnt信號至少存在經典和非經典兩條不同的信號通路，而Wnt-5a屬于非經典Wnt信號通路，既往研究Ror2具有Wnt受體結構域樣結構。有研究認為Wnt-5a經由Ror2介導的 信號通路發揮作用。
Mouse WNT-5a蛋白含有356個氨基酸殘基。
WNT-5a,Mouse 產品特性:
? WNT-5a,Mouse活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞TCF報告鑒定，純度高
? 生物活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定
? WNT-5a,Mouse產品經過SDS-PAGE 和HPLC雙層鑒定純度大于85%
WNT-5a,Mouse產品英文簡述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical" pathway. The predicted size of mouse WNT-5a is a monomeric protein containing 356 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line, and purified with a proprietary process that is distinct from the published method.
? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (Milkels AJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.
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