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Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體產品信息：

• Product nameAnti-Cyclin T1 antibody
• Description

  Sheep polyclonal to Cyclin T1

• Tested applicationsWB, IP, EMSA
• Species reactivityReacts with: Mouse, Horse, Human, Monkey

  Does not react with

  Fruit fly (Drosophila melanogaster)
• Immunogen

  Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

• General notes

   

  ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• PurityWhole antiserum
• Primary antibody notes ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
• Clonality Polyclonal
• IsotypeIgG
• Application notesEMSA: Use at an assay dependent dilution PMID 17576689.
  IP: Use at an assay dependent dilution.
  WB: 1/1000. Detects a band of approximay 87 kDa (predicted molecular weight: 81 kDa).
  
  Not yet tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

• Target

• FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
• Tissue specificityUbiquitously expressed.
• Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
• Cellular localizationNucleus.

  Anti-Cyclin T1 antibody images

  

  Western blot - Cyclin T1 antibody (ab27963)

  All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution
  Lane 1 : HeLa whole cell lysate
  Lane 2 : HeLa cell nuclear lysate
  Lane 3 : monkey Vero cell lysate
  Lane 4 : mouse kidney cell lysate
  Lane 5 : Drosophila S2 cell lysate
  Lane 6 : horse dermal cell lysate
  Secondary
  donkey anti-sheep peroxidase conjugate at 1/20000 dilution
  Predicted band size : 81 kDa
  Observed band size : 87 kDa

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